BASTARDO-FERNÁNDEZ I., CHEKRI R., NOIREAUX J., GIVELET L., LAMBENG N., DELVALLÉE A., LOESCHNER K., FISICARO P. and JITARU P., "Characterisation of titanium dioxide (nano)particles in foodstuffs and E171 additives by single particle inductively coupled plasma-tandem mass spectrometry using a highly efficient sample introduction system", Food Additives & Contaminants: Part A, 2024, 41, 8, 867‑884, DOI: 10.1080/19440049.2024.2359532.

BASTARDO-FERNÁNDEZ I., CHEKRI R., OSTER C., THOURY V., FISICARO P., JITARU P. and NOIREAUX J., "Assessment of TiO₂ (nano)particles migration from food packaging materials to food simulants by single particle ICP-MS/MS using a high efficiency sample introduction system", NanoImpact, 2024, 34, 100503, DOI: 10.1016/j.impact.2024.100503.

BUCHTA C., BENKA B., DELATOUR V., FAÉ I., GRIESMACHER A., HELLBERT K., HUGGETT J., KAISER P., KAMMEL M., KESSLER A., KESSLER HH., MÜLLER D., ROSENDAHL J., SCHEIBLAUER H., SCHWEIGER CR., ZEICHHARDT H. and COBBAERT CM., "Reference, calibration and referral laboratories – a look at current European provisions and beyond", Clinical Chemistry and Laboratory Medicine (CCLM), 2025, 63, 4, 656‑669, DOI: 10.1515/cclm-2024-1066.

CAVALIER E., FARRÉ-SEGURA J., LUKAS P., GENDEBIEN A-S., PEETERS S., MASSONNET P., LE GOFF C., BOUQUEGNEAU A., SOUBERBIELLE J-C., DELATOUR V. and DELANAYE P., "Unveiling a new era with liquid chromatography coupled with mass spectrometry to enhance parathyroid hormone measurement in patients with chronic kidney disease", Kidney International, 2024, 105, 2, 338‑346, DOI: 10.1016/j.kint.2023.09.033.

D’AGOSTINO G., OELZE M., VOGL J., GHESTEM J-P., LAFAURIE N., KLEIN O., PRÖFROCK D., DI LUZIO M., BERGAMASCHI L., JAĆIMOVIĆ R., OSTER C., IRRGEHER J., LANCASTER ST., WALCH A., RÖTHKE A., MICHALISZYN L., PRAMANN A., RIENITZ O., SARA-AHO T., CANKUR O., KUTAN D. and NOIREAUX J., "Development and application of reference and routine analytical methods providing SI-traceable results for the determination of technology-critical elements in PCB from WEEE", Journal of Analytical Atomic Spectrometry, 2024, 39, 11, 2809‑2823, DOI: 10.1039/D4JA00235K.

DEPREZ L., JOHANSEN JV., KELLER T., BUDD J., GREENBERG N., WEYKAMP C., SANDBERG S., PANTEGHINI M., CERIOTTI F., BARCZAK E., REJ R., FAUSKANGER PK., MACKENZIE F., CAMARA JE., LYLE AN., MILLER WG. and DELATOUR V., "Recommendations for assessing commutability of a replacement batch of a secondary calibrator certified reference material", Clinica Chimica Acta, 2025, 567, 120097, DOI: 10.1016/j.cca.2024.120097.

DEUMER J., SCHÜRMANN R., GAÁL A., VARGA Z., BETTIN B., VAN DER POL E., NIEUWLAND R., OJEDA D., SIKORA A., BARTCZAK D., GOENAGA-INFANTE H., NOIREAUX J., KHAKPOUR M., KORPELAINEN V. andGOLLWITZER C., "Traceable characterization of hollow organosilica beads as potential reference materials for extracellular vesicle measurements with optical techniques", Discover Nano, 2024, 19, 1, 14, DOI: 10.1186/s11671-024-03956-3.

FAUSKANGER PK., SANDBERG S., JOHANSEN J., KELLER T., BUDD J., MILLER WG., STAVELIN A., DELATOUR V., PANTEGHINI M. and STØVE B., "Quantification of Difference in Nonselectivity Between In Vitro Diagnostic Medical Devices", Biometrical Journal, 2025, 67, 1, e70032, DOI: 10.1002/bimj.70032.

GUIGUES N., UYSAL E., RAVEAU S., MAGAR J., BILLON G., KAHILUOTO J., SCHNEIDER RJ., LIV L., GENÇTÜRK TOSUN S., CANTEAU G. and LALERE B., "Insights on the validation of alternative tools for water quality monitoring: the case of on-site test kits, portable devices and continuous measuring devices", Accreditation and Quality Assurance, 2024, 29, 2, 163‑173, DOI: 10.1007/s00769-023-01570-x.

HILL S., INFANTE HG., ENTWISLE J., STREKOPYTOV S., WARD-DEITRICH C., COWEN S., RIENITZ O., ROETHKE A., GOERLITZ V., SCHULZ U., PAPE C., VOGL J., KOENIG M., JACIMOVIC R., FISICARO P., REN T., WANG S., SONG P., LI H., LINSKY M., SOBINA E., LOZANO HE., PUELLES M., YAMANI R. and HARALDSSON C., "CCQM-K160: platinum group elements in automotive catalyst", Metrologia, 2024, 61, 1A, 08011, DOI: 10.1088/0026-1394/61/1A/08011.

LASCHI S., SFRAGANO PS., TADINI-BUONINSEGNI F., GUIGUES N. and PALCHETTI I., "Development of a flow system for decentralized electrochemical analysis of heavy metals using screen-printed electrodes: the importance of sensor stability", The Analyst, 2024, 149, 16, 4239‑4249, DOI: 10.1039/D4AN00616J.

TOUZÉ S., HUBAU A., GHESTEM J-P., MOREAU P., LAFAURIE N. and NOIREAUX J., "Estimation of the uncertainty of metal content in a batch of waste printed circuit boards from computer motherboards", Waste Management, 2024, 189, 325‑333, DOI: 10.1016/j.wasman.2024.08.010.

MACÉ T., "Instrumented platform on sensor systems evaluation in a controlled environment", Gas analysis 2024, Paris (France), 30 Fébruary 2024.

MACÉ T. and WETERINGS W., "Training Course Accreditation", Training Course Accreditation, Paris (France), 30 February 2024.

DURET C., "Défis et besoins de références métrologiques pour la quantification d’hormones stéroïdiennes dans le sérum humain", Journée AFSEP, Paris (France), 13 March 2024.

BOEUF A., "Hormones stéroïdiennes & Métrologie au coeur du Concept mondial « One Health »", Séminaire de l’Académie des Technologies, Paris (France), 27 March 2024.

GUIGUES N., "Environnement, changement climatique et santé, quel apport de la métrologie ? Quelle métrologie pour les outils innovants de surveillance de la qualité de l’eau ?", Séminaire de l’Académie des Technologies, Paris (France), 27 March 2024.

CAPITAINE G., FISICARO P. and WAGENER P., "Quantification of seawater total alkalinity measurement uncertainty to support the evaluation of ocean alkalinity enhancement", EGU General Assembly 2024, Vienne (Autriche), 15 April 2024.

CAPITAINE G., ALASONATI E. and FISICARO P., "Results of the EMPIR project SapHTies: Metrology for standardised seawater pHT measurements in support of international and European climate strategies", CCQM Working Group on Electrochemical Analysis annual meeting, Sèvres (France), 22 April 2024.

MACÉ T., "Instrumented Platform on sensors evaluation in a controlled environment", 14th International Conference on Air Quality, Helsinki (Finlande), 13 May 2024.

ALASONATI E., "Metrological traceability of measurement data from nano- to small microplastics for a greener environment and food safety", EMN Pollution Monitoring Stakeholders meeting, Paris (France), 21 May 2024.

DURET C., VANEECKHOUTTE H., DELATOUR V. and BOEUF A., "Défis et besoins de références métrologiques pour la quantification d’hormones stéroïdiennes dans le sérum humain", Ecole thématique de l’AFSEP ’Une plongée captivante dans l’univers des Omics’’, Le Lavandou (France), 21 May 2024.

DURET C., VANEECKHOUTTE H., DELATOUR V., BOEUF A. et PICHON V., "Steroid hormones: Context, Challenges, and Metrological Needs in human serum for testosterone, aldosterone and 17β-estradiol", Congrès HTC-18, Louvain (Belgique), 28 May 2024.

ALASONATI E. et CARSALADE DU PONT V DE., "How to make optimisation deal with troubleshooting", 23rd International Symposium on Field- and Flow-based Separations, Nantes (France), 3 June 2024.

ALASONATI E., PAROT J., MOLSKA A., DIAZ FERNANDEZ YA., DOVERI LR., CAEBERGS T., BARTCZAK D., POUL L., SACK F., CECCALDI, A., SCHUERMANN R. et CAPUTO F., "Development and harmonisation of robust and efficient fractionation strategies for characterisation of nanomaterials in biological matrices", 23rd International Symposium on Field- and Flow-based Separations, Nantes (France), 3 June 2024.

GIANGRANDE C., "Standardisation of Measurements of Neurodegenerative Disease Biomarkers: The NEuroBioStand Project", FAAST (France Alzheimer Actualités Scientifiques & Thérapeutiques), Paris (France), 3 June 2024.

MESSAOUDI S., VANEECKHOUTTE H., DELATOUR V., VINH J., VERDIER Y., BLENNOW K., ZETTERBERG H., GOBOM J. et GIANGRANDE C., "Characterisation of candidate primary calibrators materials for P-tau measurements in biological fluids", FAAST (France Alzheimer Actualités Scientifiques & Thérapeutiques), Paris (France), 3 June 2024.

VANEECKHOUTTE H., MESSAOUDI S., HIRTZ C., LEHMANN S., JEROMIN A., VINH J., VERDIER Y., DELATOUR V. and GIANGRANDE C., "Routes to standardisation for tau protein measurement in CSF to be used in the diagnosis of neurodegenerative diseases", FAAST (France Alzheimer Actualités Scientifiques & Thérapeutiques), Paris (France), 3 June 2024.

GIANGRANDE C., "Standardisation of Measurements of Neurodegenerative Disease Biomarkers: The NEuroBioStand Project", Symposium « Biomarkers in Neurodegenerative diseases », Montpellier (France), 6 June 2024.

MESSAOUDI S., VANEECKHOUTTE H., DELATOUR V., VINH J., VERDIER Y., BLENNOW K., ZETTERBERG H., GOBOM J. and GIANGRANDE C., "Characterisation of candidate primary calibrators materials for P-tau measurements in biological fluids", Symposium « Biomarkers in Neurodegenerative diseases », Montpellier (France), 6 June 2024.

VANEECKHOUTTE H., MESSAOUDI S., HIRTZ C., LEHMANN S., JEROMIN A., VINH J., VERDIER Y., DELATOUR V. and GIANGRANDE C., "Routes to standardisation for tau protein measurement in CSF to be used in the diagnosis of neurodegenerative diseases", Symposium « Biomarkers in Neurodegenerative diseases », Montpellier (France), 6 June 2024.

GUIGUES N., LEPOT B., CONSTANTIN B., LECCIA F. and BOUBETRA A., "Use of 5 years of Proficiency Testing for water sampling and in situ non-conservative measurements to estimate measurement uncertainty including sampling", Eurachem Workshop Quality Assurance in Chemical, Medical and Microbiological Laboratories, Vilnius (Lithuanie), 13 June 2024.

GIANGRANDE C., VANEECKHOUTTE H., DELATOUR V., VINH J., VERDIER Y., VANMECHELEN E., KOERTVELYESSY P., BLENNOW K., ZETTERBERG H. and GOBOM J., "NEuroBioStand project: a European metrological research framework for standardising p-tau measurements in plasma", AAIC 2024, Philadelphie (Etats-Unis), 28 August 2024.

DURET C., VANEECKHOUTTE H., DELATOUR V., BOEUF A. and PICHON V., "Analytical developments towards new metrological references for the quantification of testosterone, aldosterone and 17β-estradiol in human serum", Congrès PBA 2024, Genève (Suisse), 9 September 2024.

CAPITAINE G., FISICARO P. and WAGENER P., "Metrological concepts applied to total alkalinity measurements in support of ocean alkalinity enhancement assessment", ICOS International Conference, Versaille (France), 10 September 2024.

MESSAOUDI S., VANEECKHOUTTE H., DELATOUR V., VINH J., VERDIER Y., BLENNOW K., ZETTERBERG H., GOBOM J. and GIANGRANDE C., "Characterisation of candidate primary calibrators materials for P-tau measurements in biological fluids", SMAP 2024, Lille (France), 16 September 2024.

ALASONATI E., "From Qualitative to Quantitative Measurement of Small Microplastics Using Multi-Detector Field Flow Fractionation Coupled Offline to Microscopy and Raman Spectroscopy", MICRO2024: Plastic pollution from micro to nano, Lanzarote (Espagne), 23 September 2024.

ALASONATI E., "Towards Nanoplastic Reference Materials Representative of Partially Degraded/Naturally Aged Samples in Complex Food and Environmental Matrices", MICRO2024: Plastic pollution from micro to nano, Lanzarote (Espagne), 23 September 2024.

BOEUF A. and GIANGRANDE C., "Calibration of RMPs with peptides vs recombinant proteins: Example of procalcitonin and ptau", Workshop CCQM PAWG, Berlin (Allemagne), 7 October 2024.

MACÉ T., "Mise en place du nouveau coefficient d’absorption de l’ozone", Journées Techniques de l’Air, Ajaccio (France), 7 October 2024.

GUIGUES N., ZUG M. and HÉTEAU A., "Les démos de mon réseau d’eau", Master classe : Comment mesure-t-on la qualité de l’eau ?, En ligne, 11 October 2024.

DELATOUR V., "Presentation of the COMET project", EQALM Symposium, Vienne (Autriche), 17 October 2024.

DELATOUR V., "Health event", EURAMET Workshop “Metrology for regulation”, Visioconférence, 21 October 2024.

DELATOUR V., "The central role of commutability of materials used for trueness verification and postmarket surveillance", UMK Congress 2024, Bydgoszcz (Pologne), 29 November 2024.

ALASONATI E. and BENISMAIL N., "Inside the ILC Data for ISO 16094-2: Microplastics in Water via Vibrational Spectroscopy", Stakeholder Workshop PlasticTrace and COST Action Priority, 14 December 2024.

Since the beginning of the industrial revolution, the concentration of carbon dioxide (CO₂) in the atmosphere has increased by 50% due to human activities. Each year, the ocean absorbs 26% of CO₂ emissions. However, this absorption leads to ocean acidification and changes in the carbonate system. This, by affecting the stability of calcium carbonate mineral forms, affects marine biodiversity. Oceanographic measurements are essential for observing the oceanic carbonate system, supporting decision-making regarding mitigation and adaptation strategies. Monitoring acidification relies on accurate measurements of variables such as pH_T and total alkalinity (TA). To ensure data quality, international recommendations and best practice guidelines have been established, such as those from the Global Ocean Acidification Observing Network (GOA-ON), which sets data quality objectives.

The thesis work aims to support oceanographers with metrological tools to ensure the comparability of pH_T and TA measurement results. Metrology, including traceability, validation of measurement procedures, and uncertainty assessment, is crucial for obtaining reliable and comparable results, enabling the evaluation of long-term trends in ocean acidification.

For both parameters, reference materials have been developed and tested in inter-laboratory comparisons, allowing the evaluation of the precision and trueness of measurement methods. The uncertainty budgets of pH_T and TA measurements have also been studied using two approaches of uncertainty quantification.

Finally, improvements to the metrological traceability chains are presented.

ocean acidification, carbonate system, total pH, total alkalinity, metrology, traceability

Full document (EN): 

Titanium dioxide (TiO₂) appears to be one of the most employed pigments in the industry due to its specific structural, optical, electronic and photocatalytic properties. It is a solid, crystalline, white and bright, odourless inorganic substance in the form of a powder with particles size ranging from the nanometre (nm) to the micrometre (µm) scale. TiO₂ has been used for decades as a coloring additive in food (E171), especially in candies, chewing gums and frostings. However, since the first report regarding the presence of TiO₂ having particles with size < 100 nm (NPs), the concern about the toxicity of E171 increased, which led recently (2022) to an European Regulation banning the use of this additive in food in the European Union (EU). Food-grade TiO₂ is, however, still used in food industry outside the EU and in food packaging all around the world, thus the question of the migration of TiO₂ particles in food is raised. Reliable analytical methods for the characterisation of TiO₂ are still necessary to assess human exposure by ingestion of food contaminated with E171 and TiO₂-based food packaging.

The Ph.D. project addressed primarily the development and various applications of a new approach for the characterisation (detection and quantification) of NPs in complex matrices such as foodstuffs and food simulants by inductively coupled plasma-mass spectrometry in combination with the single particle approach (spICP-MS). Among the relatively large panel of available techniques for the characterisation of (nano)materials, spICP-MS shows promising features since it allows the rapid screening and analysis of the individual particles in the sample, either in terms of concentration (down to ng/L levels) and size (from ~ 30 to 900 nm).

The first part of the thesis describes the development and validation of a novel method based on spICP-MS/MS using a high efficiency sample introduction system (APEX™) for the characterisation and quantification of TiO₂ NPs in food simulants. The accuracy was demonstrated for both particle size determination and quantitative measurements, and the method’s uncertainty was also calculated. The APEX™ proved to be an excellent approach to avoid matrix effects and increase the sensitivity of the method allowing the detection of particles as small as 12 nm.

The second part of the thesis consisted in the application of the developed method for two different studies. On the one hand, the APEX™-spICP-MS/MS method was used for the assessment of TiO₂ NPs migration from food packaging into food simulants upon storage, showing very low migration yield of TiO₂ NPs from food packaging in standard contact conditions of storage. On the other hand, the method was applied to the characterisation of TiO₂ NPs from E171 in food samples. The main objective was to assess the feasibility of the method for the analysis of real food matrices, especially regarding the detection and quantification of the nanoparticulate fraction (% of particles having a diameter < 100 nm). In addition, a pilot inter-comparison study on the characterisation of E171 additive was performed using different techniques at different laboratories in France and abroad.

The last part of the thesis consisted of the development of an in-house approach (based on python code) for spICP-MS data treatment to assess the main parameters influencing the method uncertainty related to the characterisation of TiO₂ NPs by spICP-MS.

inductively coupled plasma mass spectrometry, titanium oxide, TiO₂ nanoparticles, single particle ICP-MS/MS, high efficiency sample introduction system, E171 additive, foodstuffs, migration and food packaging

Full document (EN): TEL-04678080

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GIANGRANDE C., DELATOUR V., ANDREASSON U., BLENNOW K., GOBOM J. et ZETTERBERG H., "Harmonization and standardization of biofluid‐based biomarker measurements for AT(N) classification in Alzheimer’s disease", Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring, 15, 3, 2023, e12465, DOI: 10.1002/dad2.12465.

GIANGRANDE C., VANEECKHOUTTE H., BOEUF A., LALERE B., HIRTZ C., LEHMANN S., QUAGLIA M. et DELATOUR V., "Development of a candidate reference measurement procedure by ID-LC-MS/MS for total tau protein measurement in cerebrospinal fluid (CSF)", Clinical Chemistry and Laboratory Medicine (CCLM), 61, 7, 2023, 1235‑1244, DOI: 10.1515/cclm-2022-1250.

GIANGRANDE C., VANEECKHOUTTE H., HIRTZ C., LEHMANN S., JEROMIN A. et DELATOUR V., "Routes to standardisation for tau protein measurement in CSF", Alzheimer’s & Dementia, 19, S14, 2023, e076172, DOI: 10.1002/alz.076172.

HUBER MJ., IVLEVA NP., BOOTH AM., BEER I., BIANCHI I., DREXEL R., GEISS O., MEHN D., MEIER F., MOLSKA A., PAROT J., SØRENSEN L., VELLA G., PRINA-MELLO A., VOGEL R. et CAPUTO F., "Physicochemical characterization and quantification of nanoplastics: applicability, limitations and complementarity of batch and fractionation methods", Analytical and Bioanalytical Chemistry, 415, 15, 2023, 3007‑3031, DOI: 10.1007/s00216-023-04689-5.

LAMAS B., CHEVALIER L., GAULTIER E., CARTIER C., WEINGARTEN L., BLANC X., FISICARO P., OSTER C., NOIREAUX J., EVARISTE L., BREYNER NM. et HOUDEAU E., "The food additive titanium dioxide hinders intestinal production of TGF-β and IL-10 in mice, and long-term exposure in adults or from perinatal life blocks oral tolerance to ovalbumin", Food and Chemical Toxicology, 179, 2023, 113974, DOI: 10.1016/j.fct.2023.113974.

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MIRMONT E., BŒUF A., CHARMEL M., LALÈRE B. et LARDY-FONTAN S., "Validation of an isotope dilution mass spectrometry (IDMS) measurement procedure for the reliable quantification of steroid hormones in waters", Analytical and Bioanalytical Chemistry, 415, 16, 2023, 3215‑3229, DOI: 10.1007/s00216-023-04698-4.

RUHAAK LR., ROMIJN FPHTM., BEGCEVIC BRKOVIC I., KUKLENYIK Z., DITTRICH J., CEGLAREK U., HOOFNAGLE AN., ALTHAUS H., ANGLES-CANO E., COASSIN S., DELATOUR V., DEPREZ L., DIKAIOS I., KOSTNER GM., KRONENBERG F., LYLE A., PRINZING U., VESPER HW. et COBBAERT CM., "Development of an LC-MRM-MS-Based Candidate Reference Measurement Procedure for Standardization of Serum Apolipoprotein (a) Tests", Clinical Chemistry, 69, 3, 2023, 251‑261, DOI: 10.1093/clinchem/hvac204.

SANDBERG S., FAUSKANGER P., JOHANSEN JV., KELLER T., BUDD J., GREENBERG N., REJ R., PANTEGHINI M., DELATOUR V., CERIOTTI F., DEPREZ L., CAMARA JE., MACKENZIE F., LYLE AN., VAN DER HAGEN E., BURNS C., GREG MILLER W., et FOR THE IFCC WORKING GROUP ON COMMUTABILITY IN METROLOGICAL TRACEABILITY, "Recommendations for Setting a Criterion and Assessing Commutability of Sample Materials Used in External Quality Assessment/Proficiency Testing Schemes", Clinical Chemistry, 69, 11, 2023, 1227‑1237, DOI: 10.1093/clinchem/hvad135.

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TSE CY., ZHANG L., DAHYA K., POKUAH F., SUGAHARA O., DELATOUR V., DANILENKO U. et VESPER HW., "B-185 High Throughput ID-LCMSMS Method for Simultaneous Quantitation of Monosaccharides in Human Serum", Clinical Chemistry, 69, Supplement_1, 2023, hvad097.516, DOI: 10.1093/clinchem/hvad097.516.

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WIELGOSZ R., FORGAN B., DEL CAMPO MALDONADO D., REA A., WOOLLIAMS E., FULFORD J., MADONNA F.,  WHETSTONE J., DECOLA P., VERMEULEN A., FLORES E. and RUEDI I., "BIPM-WMO  Metrology for Climate Action Workshop - 26-30 September 2022", Report BIPM (2023/03) & WMO (142), 88 pages, 2023, DOI: 10.59161/Rapport202303.

DELATOUR V., "IFCC C-BM second commutability study on PTH", Joint meeting on PTH 1-84 reference materials, methods and comparisons, BIPM, Sèvres, France, 8 February 2023.

BASTARDO-FERNÁNDEZ I., CHEKRI R., NOIREAUX J., FISICARO P. et JITARU P., "Development of a novel method based on single particle icp-ms/ms and a high sensitivity sample introduction (apex) system for the characterization of titanium dioxyde nanoparticles in food simulants", European Winter Conference on Plasma Spectrochemistry, Ljubljana, Slovénie, 29 January-3 February 2023.

NOIREAUX J., OSTER C., HUBAU A., TOUSE S., DI LUZIO M., D’AGOSTINO G. et GHESTEM J-P., "Improving valuable metals recycling : best digestion method for retrieving Technology Critical Elements", European Winter Conference on Plasma Spectrochemistry, Ljubljana, Slovénie, 29 January-3 February 2023.

BASTARDO-FERNÁNDEZ I., ZAOUALI J., NOIREAUX J., CHEKRI R., FISCHER N., JITARU P. et FISICARO P., "Assessment of the measurement uncertainty related to nanoparticles characterization by sp-icp-ms using an in-house data treatment software", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

FILTZ J-R., AMALRIC L., GUIGUES N., NÄYKKI T., MARTIN V. et FISICARO P., "Metrology in the loop of the circular economy - Water Metrology - Round Table", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

FOUQUEAU A., "Towards a better monitoring of ammonia and greenhouse gases emissions from livestock production: the quantiAGREMI project", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

GUIGUES N., "EURAMET / EMN for Pollution Monitoring", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

MACÉ T., "Towards an unbroken SI-traceable calibration chain for VOCs at monitoring stations, Preparation of reference gas mixtures (RGMs) of climate relevant VOCs", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

NOIREAUX J., OSTER C., LAMAS B. et HOUDEAU E., "Accurate quantification of the bioaccumulation of titanium dioxide particles", Congrès International de Métrologie (CIM-2023), Lyon, France, 7-10 March 2023.

DELATOUR V., "Traçabilité métrologique des résultats et contrôle de qualité congrès", Congrès BIOMEDJ, Paris, France, 9 March 2023.

ALASONATI E., GIOVANNOZZI A., CHIVAS-JOLY C., FELTIN N., FISICARO P. et ROPERS M-H., "Metrological traceability of nano and microplastic measurement data: focus on the use of the multi-detector FFF coupled off-line with microscopy and Raman spectroscopy French Conference on Separative Sciences and Couplings", SEP23, Paris, France, 28-30 March 2023.

NOIREAUX J., "Preparing electronic wastes for the chemical analysis of Technology Critical Elements", Stakeholder workshop MetroCycleEU, Virtual meeting, March 2023.

BESCOND A., "Évaluation des performances d’instruments portables de carbone suie avec une plateforme expérimentale en environnement contrôlé", 36^e Congrès français sur les aérosols (CFA-2023), Asfera, Paris, France, 15-16 March 2023.

GAIE-LEVREL F., "Métrologie des aérosols - Applications dans les domaines de la qualité de l’air, des nanotechnologies et de la santé", 36^e Congrès français sur les aérosols (CFA-2023), Asfera, Paris, France, 15-16 March 2023.

OUF F.-X., GAIE-LEVREL F., CHAZELET S., FAVRE G., FELTIN N., FERRÉ V., GODEFERT V., MAST J., NOIREAUX J., PACAULT S., POISSON X. et WOUTERS C., "Relargage de nano-objets, leurs agrégats et agglomérats depuis les masques : ambitions et objectifs scientifiques du Projet RENAAME", 36^e Congrès français sur les aérosols (CFA-2023), Asfera, Paris, France, 15-16 March 2023, DOI: 10.25576/ASFERA-CFA2023-33869.

MACÉ T., "Développement d’un étalon de référence pour assurer la traçabilité des mesures de NH3", Journées Techniques de l’Air, Strasbourg, France, 22-23 March 2023.

MACÉ T., "Retour d’expérience sur les CIL LCSQA-LNE/AASQA", Journées Techniques de l’Air, Strasbourg, France, 22-23 March 2023.

GIANGRANDE C., "Activités du LNE dans le domaine des maladies Neurodégénératives", AMI Brain-STORM, Paris, France, 29 March 2023.

DURET C., VANEECKHOUTTE H., LALÈRE B., BONNAUD B., DELATOUR V., BOEUF A. et PICHON V., "Les hormones stéroïdiennes : contexte, enjeux et besoins métrologiques dans le sérum humain", Congrès Francophone sur les Sciences Séparatives et les Couplages 2023, Paris, France, 29-30 March 2023.

CAPITAINE G., "L’intérêt de la métrologie pour le suivi de l’Alcalinité Totale des eaux marines", Congrès de l’école doctorale « Sciences de l’environnement – Université Aix-Marseille », 3-4 May 2023.

BOEUF A., VANEECKHOUTTE H., HUYNH H-H., GIANGRANDE C., VELLY L., HAUSFATER P., CREAGH-BROWN B., FALINSKA AM., DEMKOW U., ANAGNOSTOPOULOU V., TSATSANIS C., CRISTOL J-P., PIERONI L., HUGGETT JF. et DELATOUR V., "Standardisation of PCT assays: Update on status, needs and feasibility", EuroMedLab, Rome, Italie, 21-25 May 2023.

DELATOUR V., "Harmonisation of Procalcitonin as a sepsis biomarker: problems and solutions”, Session “Diagnostic Challenges in Sepsis", EuroMedLab, Rome, Italie, 21-25 May 2023.

GIANGRANDE C., VANEECKHOUTTE H., HIRTZ C., LEHMANN S., JEROMIN A. et DELATOUR V., "Routes to standardisation for tau protein measurement in CSF to be used in the diagnosis of neurodegenerative diseases", EuroMedLab, Rome, Italie, 21-25 May 2023.

GUIGUES N., BONNAUD B., FISICARO P. et GUMUCHIAN D., "European Metrology Network for Pollution Monitoring POLMO", Eurachem Workshop 2023, Bern-Wabern, Suisse, 22-23 May 2023.

GUIGUES N., "Need for the validation of on-site test kits, portable devices and continuous measuring devices for water quality monitoring", Eurachem Workshop 2023, Bern-Wabern, Suisse, 22-23 May 2023.

GIANGRANDE C., "Mieux diagnostiquer les maladies neurodégénératives : quand la métrologie fait la différence !", Les mardis de la chimie de Sorbonne Université, Paris, France, 13 June 2023.

GUIGUES N., "The European Metrology Network for Pollution Monitoring – PolMo.", PARC Hub FR, 16 June 2023.

DELATOUR V., "What is commutability and why is it important?", InterQC Topics, Virtual meeting, 15 July 2023.

GIANGRANDE C., VANEECKHOUTTE H., HIRTZ C., LEHMANN S., JEROMIN A. et DELATOUR V., "Routes to standardisation for tau protein measurement in CSF", Alzheimer’s Association International Conference, Amsterdam, Pays-Bas, 16-20 July 2023.

TSE CY., ZHANG L., DAHYA K., POKUAH F., SUGAHARA O., DELATOUR V., DANILENKO U. et VESPER HW., "High Throughput ID-LCMSMS Method for Simultaneous Quantitation of Monosaccharides in Human Serum", AACC Annual Scientific Meeting & Clinical Lab Expo, Anaheim, Etats-Unis, 23-27 July 2023.

CAPITAINE G., "Le système des carbonates océanique : La qualité des mesures d’Alcalinité Totale et de pH pour le suivi de l’acidification des oceans", MOOSE GE 2023, August 2023.

FISICARO P., "The role of metrology into the value chain of ocean observations", IAPWS Symposium, Turin, Italie, 3-8 September 2023.

ALASONATI E., NOIREAUX J., CHIVAS-JOLY C., FELTIN N., FISICARO P., LALÈRE B. et GIOVANNOZZI A., "Metrological characterisation of nanoparticles in FOOD products and complex matrices using AF4-MALS-ICPMS and sp-ICPMS", EMN Food “Metrological determination of micro and nano contaminants in food”, Bern, Switzerland (remote participation), 5 September 2023.

DELATOUR V., "Insights into the development of primary reference materials: what are the hurdles primary reference material manufacturers have to overcome?", Siemens 3rd Virtual TRSEG Assay Development Annual Conference, Virtual meeting, 28 September 2023.

CAPITAINE G., "Inter-laboratory comparison on a reference material for seawater spectrophotometric pHT measurements", Metrosea, Valetta Campus, Malta, October 2023.

GUIGUES N., "Valider les outils utilisant l’ADNe : pourquoi et comment", GT-DCE, 12 October 2023.

DELATOUR V., "Metrology input for post-market surveillance of IVD tests", EQALM Symposium, Lyon, France, 19 October 2023.

GUIGUES N., "State of the art on metrology and standardisation needs to support EU regulations.", Euramet & Welmec Metrology for regulationn – Open public Consultation, Virtual meeting, 23 October 2023.

TSE CY., ZHANG L., DAHYA K., POKUAH F., DANILENKO U., DELATOUR V. et VESPER HW., "Comparison between ID-LC/MS/MS Serum Monosaccharides Method and Enzymatic Methods on Glucose Measurements", Diabetes Technology Meeting, Virtual meeting, 3-4 November 2023.

GUIGUES N., "Validation indépendante des outils innovants de mesure de la qualité de l’eau : application aux méthodes ADNe", Journées du réseau ADN-O, 29-30 November 2023.

BASTARDO-FERNÁNDEZ I., CHEKRI R., NOIREAUX J., FISICARO P. et JITARU P., "Development of a single particle ICP-MS method using a high efficiency sample introduction system (APEXTM) for the characterisation of titanium dioxide nanoparticles in foodstuffs", IMEKOFOOD 2023, Maisons-Alfort, France, 25-27 October 2023.

NOIREAUX J., "Présentation des activités de métrologie sur les nanoparticules au LNE", Vidéo du BIPM réalisée à l’occasion du world Metrology Day, lien: https://www.youtube.com/watch?v=GTXMjUoLiFk&list=PL-vj-3_a7wTAKMXIKtU8jSY7bNf5TI_6s&index=10

OUF F.-X. , CHAZELET S., FAVRE G., FELTIN N., LEMARINIER V., GODEFERT V., LAMBENG N., MAST J., NOIREAUX J., OSTER C., PACAULT S., POISSON X. et WOUTERS C., "Relargage de nano-objets, leurs agrégats et agglomérats depuis les masques (Projet RENAAME) : Validation d'une méthode de "criblage" pour l'identification des éléments Ti et Ag dans les masques", 37^e Congrès français sur les aérosols (CFA-2024), Asfera, Paris, France, 19-20 March 2024, DOI: 10.25576/ASFERA-CFA2024-38823.

Sepsis is a syndrome caused by a dysregulated immune system response to an infection (bacterial, viral, fungal), leading to organ failure. In 2017, 48.9 million cases of sepsis were reported worldwide, resulting in 11 million deaths. Early and accurate diagnosis is critical for implementing a therapeutic treatment and improving patient survival. Currently, sepsis diagnosis is based on clinical evaluation of the patient, which can be influenced by nonspecific symptoms. The individual quantification of biomarkers such as procalcitonin (PCT) or C-reactive protein (CRP) using immunological methods serves as an aid in diagnosis. Since quantifying a single biomarker may lack specificity, simultaneous monitoring of a panel of biomarkers appears to be a significant advantage for early diagnosis.

In the present work, a list of nine protein biomarkers of interest for sepsis diagnosis was established in collaboration with clinicians and in vitro diagnostic manufacturers. The objective was then to develop a targeted proteomic method using high-resolution mass spectrometry (HRMS) to enable specific, sensitive, and multiplexed quantification of these biomarkers in routine, in clinical serum samples.The proteins targeted in this study have different molecular masses and serum concentrations, with a dynamic range of approximately 105 in healthy patients' serum. Thus, the quantification of these proteins by HRMS is challenging. The first step was to select two proteotypic peptides per protein, considering various criteria such as molecular mass or amino acid composition. These peptides were then used to optimise high-performance liquid chromatography conditions (chromatographic columns, mobile phases, elution gradients), ionisation, fragmentation, and HRMS analysis, as well as sample preparation workflow (protein precipitation, enzymatic digestion, peptide extraction), allowing specific and sensitive quantification of the targeted proteins. These developments led to the identification of increasingly selective protocols, resulting in a decrease in the limits of quantification. Thus, three methods were developed: i) the first targeting five proteins and meeting clinical (covering physiological and pathological concentrations) and metrological (metrological traceability) needs, ii) the second targeting eight proteins and meeting the clinical need for a highly multiplexed method, and iii) the last targeting PCT, allowing comparison with an existing reference method.To ensure reliable quantification and traceability of measurement results to SI units, a protein-based calibration was selected for proteins with existing certified reference materials (recombinant proteins. In contrast a peptide-based calibration was selected for the other proteins. Therefore, it was necessary to rigorously characterise peptide standards (purity assessment and quantification through amino acid analysis) during this work.

The validation of the first method and the evaluation of measurement uncertainties for the quantification of the five biomarkers in human serum, followed by the analysis of clinical samples, allowed the assessment of its potential implementation for early and specific diagnosis of sepsis in routine practice. Finally, the development of a robust LC-HRMS/MS method and rigorously characterised standards represents a significant advancement in establishing metrological references.

Multiplex quantification, ID-LC-hrMS/MS, sepsis, biomarker, metrology, sample preparation

BADRICK T., MILLER W.G., PANTEGHINI M., DELATOUR V., BERGHALL H., MACKENZIE F. and JONES G., “Interpreting EQA-Understanding Why Commutability of Materials Matters”, Clin Chem. ,2022, 68, 4, 494-500, DOI: 10.1093/clinchem/hvac002.

BAUDIN B., BOEUF A., DELATOUR V., DESMONS A., GOUGET B., GRUSON D., PEOC'H K., SAPIN V., VASSAULT A., VAUBOURDOLLE M., “Reports of Euromedlab 2021, in 2022”, Ann Biol Clin, 2022, Paris, 80, 3, 274-286, DOI: 10.1684/abc.2022.1728.

BOEUF A., HUYNH H.-H., DERBEZ M., GIANGRANDE C., LALÈRE B., VASLIN-REIMANN S. and DELATOUR V., “M260 Towards the standardization of PCT assays?”, Clinica Chimica Acta, 2022, 530, Suppl.1, S431.

DELEEBEECK L., SNEDEN A. and STOICA D., “Reconciling the pHe measurements of bioethanol: pHabs measurements of buffered 50-50 wt% water-ethanol mixtures”, Anal Chim Acta X, 2022, 10, DOI: 100085. doi: 10.1016/j.acax.2022.100085.

EVARISTE L., GAULTIER E., CARTIER C., NOIREAUX J., CHASSAING B., HOUDEAU E. and LAMAS B., “P09-08 Mice exposed to food-grade titanium dioxide from in utero life to adulthood show sex-specific gut microbiota and metabolic disorders which are aggravated under Western-diet”, Toxicology Letters, 2022, 368, Suppl. S153, ISSN 0378-4274, DOI: 10.1016/j.toxlet.2022.07.429.

FOUQUEAU A., POURCHEZ J., LECLERC L., PEYRON A., MONTIGAUD Y., VERHOEVEN P., BESCOND A., MACÉ T., THOMAS D., CHARVET A., BOURROUS S., GHIJSELINGS M., HARS P., POLYN F. and GAIE-LEVREL F., “An inter-laboratory comparison between particle and bacterial filtration efficiencies of surgical masks in the COVID-19 pandemic context”, Aerosol and Air Quality Research, 2022, 23, 2, 220252, DOI: 10.4209/aaqr.220252.

GUIGUES N., CHABROL J., LAVAUD P., RAVEAU S., MAGAR J., LALÈRE B. and VASLIN REIMANN S., “Assessing the performances of on-line analyzers can greatly improve free chlorine monitoring in drinking water”, Accreditation and quality assurance, 2022, 27, 1, 43-53, 11, DOI: 10.1007/s00769-021-01488-2.

GUIGUES N., LARDY-FONTAN S., VILLENEUVE A., MANSUIT P., WELSCH S. and LALÈRE B., “Passive sampling as an alternative strategy to monitor metals and PAHs trends at an upstream and rural catchment: a French case study”, SN Applied sciences, 2022, 4, 4, 104, DOI: 10.1007/s42452-022-04990-5.

HUYNH H.-H., DELATOUR V., DERBEZ-MORIN M., LIU Q., BOEUF A. and VINH J., “Candidate High-Resolution Mass Spectrometry-Based Reference Method for the Quantification of Procalcitonin in Human Serum Using a Characterized Recombinant Protein as a Primary Calibrator”, Anal Chem., 2022, 94, 10, 4146-4154, DOI: 10.1021/acs.analchem.1c03061.

IVANOVA V., OSTER C., SURLEVA A. and FISICARO P., “Comparative evaluation of methods for quantification of mercury at trace level in aquatic biota samples as a bio-indicator”, Journal of Chemical Technology and Metallurgy, 2022, 57, 2, 328-341.

JONES G.R.D., DELATOUR V. and BADRICK T., “Metrological traceability and clinical traceability of laboratory results - the role of commutability in External Quality Assurance”, Clin Chem Lab Med., 2022, 60, 5, 669-674, DOI: 10.1515/cclm-2022-0038.

JOSEPHS R. D., LIU Q., MARTOS G., BEDU M., DAIREAUX A., CHOTEAU T., WESTWOOD S., WIELGOSZ R. I., NAMMOONNOY J., ZHANG W., YONG S., LIU H., CHEN Y., NG C. Y., LU T., WANG J., LEUNG H. W., TEO T. L., GONG X., DAI X., XIA W., FENG L., XIE J., PENG T., FANG X., WU L., LI C., SONG J., LI M., LI H., BELTRÃO P. J., NARESSI SCAPIN S. M., BACILA SADE Y., BAHADOOR A., STOCKS B. B., THIBEAULT M.-P., MELANSON J. E., GIANGRANDE C., DELATOUR V., BOEUF A., VANEECKHOUTTE H., OHLENDORF R., O'CONNOR G., HENRION A., FUNG W.-H., WONG Y.-T., SAIKUSA K., YAMASAKI T., KINUMI T., ÖZTUG M., SABAN E., AKGÖZ M., QUAGLIA M., GROVES K., CLARKSON C., DRINKWATER G. and RUPÉREZ CEBOLLA D., “Key comparison study on peptide purity - hexapeptide of HbA0”, Metrologia, 2022, 59, 08013, DOI: 10.1088/0026-1394/59/1A/08013.

JOSEPHS R. D., LIU Q., MARTOS G., BEDU M., DAIREAUX A., CHOTEAU T., WESTWOOD S., WIELGOSZ R. I., NAMMOONNOY J., ZHANG W., YONG S., LIU H., CHEN Y., NG C. Y., LU T., WANG J., LEUNG H. W., TEO T. L., ZHAI R., DAI X., CHU Z., FANG X., PENG T., XIE J., MI W., ZHU M., LIU Y., LI M., WU L., LI H., BELTRÃO P. J., NARESSI SCAPIN S. M., BACILA SADE Y., BAHADOOR A., STOCKS B. B., THIBEAULT M.-P., MELANSON J. E., GIANGRANDE C., DELATOUR V., BOEUF A., VANEECKHOUTTE H., OHLENDORF R., O'CONNOR G., HENRION A., SAIKUSA K., KINUMI T., ÖZTUG M., SABAN E., AKGÖZ M., QUAGLIA M., GROVES K., CLARKSON C., DRINKWATER G. and RUPÉREZ CEBOLLA D., “Key comparison study on peptide purity - glycated hexapeptide of HbA1c”, Metrologia, 2022, 59, 08006, DOI: 10.1088/0026-1394/59/1A/08006.

KRIEF S., IGLESIAS-GONZALEZ A., APPENZELLER B., SPIRHANZLOVA P. and LARDY-FONTAN S., “Chimpanzee exposure to pollution revealed by human biomonitoring approaches”, 2022, Ecotoxicology and Environmental Safety, 233, 113341, DOI: 10.1016/j.ecoenv.2022.113341.

MATHON B., FERREOL M., TOGOLA A., LARDY-FONTAN S., MAZELLA N. and MIEGE C., “Polar organic chemical integrative samplers as an effective tool for chemical monitoring of surface waters - Results from one-year monitoring in France”, Science of The Total Environment, 2022, 824, 153549, DOI:  10.1016/j.scitotenv.2022.153549.

MI W., JOSEPHS R.-D., MELANSON J.-E., DAI X., WANG Y., ZHAI R., CHU Z., FANG X., THIBEAULT M.-P., STOCKS B.-B., MEIJA J., BEDU M., MARTOS G., WESTWOOD S., WIELGOSZ R.-I., LIU Q., TEO T.-L., LIU H., TAN Y.-J., ÖZTUĞ M., SABAN E., KINUMI T., SAIKUSA K., SCHNEIDER R.-J., WELLER M.-G., KONTHUR Z., JAEGER C., QUAGLIA M., MUSSELL C., DRINKWATER G., GIANGRANDE C., VANEECKHOUTTE H., BOEUF A., DELATOUR V., LEE J.-E., O'CONNOR G., OHLENDORF R., HENRION A., BELTRÃO P.-J., NARESSI SCAPIN S.-M. and SADE Y.-B., “PAWG Pilot Study on Quantification of SARS-CoV-2 Monoclonal Antibody - Part 1”, Metrologia, 2022, 59, 08001, DOI: 10.1088/0026-1394/59/1A/08001.

MIRMONT E., BOEUF A., CHARMEL M., LAPREVOTE O., LARDY-FONTAN S. and LALÈRE B., “Overcoming matrix effects in quantitative liquid chromatography–mass spectrometry analysis of steroid hormones in surface waters”, Rapid Communications in Mass Spectrometry, 2022, 36, 2, e9154, DOI: 10.1002/rcm.9154.

PHILIPP R., LALERE B., GANTOIS F. and SANCHEZ C., “Supplementary comparison study - measurement capabilities for the quantification of ethanol in water”, Metrologia, Technical suppl.,  2022, 59, 1A, 08015, DOI: 10.1088/0026-1394/59/1A/08015.

ROMSHOO B., PÖHLKER M., WIEDENSOHLER A., PFEIFER S., SATURNO J., NOWAK A., CIUPEK K., QUINCEY P., VASILATOU K., ESS M. N., GINI M., ELEFTHERIADIS K., ROBINS C., GAIE-LEVREL F. and MÜLLER T., “Importance of size representation and morphology in modelling optical properties of black carbon: comparison between laboratory measurements and model simulations”, Atmospheric Measurement Techniques, 2022, 15, 6965-6989, DOI: 10.5194/amt-15-6965-2022.

VASILATOU K., WÄLCHLI C., IIDA K., HORENDER S., TRITSCHER T., HAMMER T., RISSLER J., GAIE-LEVREL F., SAKURAI H. and AUDERSET K., “Extending traceability in airborne particle size distribution measurements beyond 10 μm: Counting efficiency and unit-to-unit variability of four aerodynamic particle size spectrometers (APS 3321)”, Aerosol Science and Technology, 2022, 57, 24-34, DOI: 10.1080/02786826.2022.2139659.

WANG J.-L., ALASONATI E., FISICARO P. and BENEDETTI M.-F., “Titanium and Titanium dioxide (nano-) particles in small watersheds under different land-uses”, Journal of Hazardous Materials, 2022, 422, DOI:  126695, DOI: 10.1016/j.jhazmat.2021.126695.

DERBEZ-MORIN M., DELATOUR V., FENAILLE F., LALERE B., VASLIN-REIMANN S., BOEUF A. and BECHER F., “Development of a multiplexed LC-HRMS/MS quantification method of nine sepsis protein biomarkers in human serum”, Rencontres du Club Jeune SFSM, Pomeys, France, 7-11 March 2022.

ALASONATI E., LARDY-FONTAN S., CHIVAS-JOLY C., FELTIN N. and FISICARO P., “LNE activities on the characterisation of the nano and microplastics in the frame of the French and European metrology strategy”, Workshop CCQM sur les microplastiques 2022, Online conference, 5 April 2022.

BOEUF A., HUYNH H.-H., DERBEZ M., GIANGRANDE C., VASLIN-REIMANN S., LALERE B., VINH J. and DELATOUR V., “Towards the standardization of PCT assays? Status, needs and feasibility”, EuromedLab, Munich, Germany, 11-15 April 2022.

DELATOUR V., “The importance of demonstrating commutability of reference materials and EQA materials”, Congrès IFCC, Munich, Germany, 13 Avril 2022.

BESCOND A., MACE T. and GAIE-LEVREL F., « Développement d'un aérosol atmosphérique modèle en environnement contrôlé pour la détermination des performances métrologiques de systèmes capteurs », 35ème Congrès Français sur les Aérosols, Paris, France, 9-10 May 2022.

BASTARDO FERNÁNDEZ I., CHEKRI R., NOIREAUX J., FISICARO P.and JITARU P., “Studying analytical parameters in the characterization of TiO₂ nanoparticles by Single Particle ICP MS”, SPECTRATOM 2022, Pau, France, 10-13 May 2022.

ALASONATI E., COULIBALY C. A., DIOP N. R., BREYSSE C., LORIOT C., FELTIN N., CHIVAS-JOLY C., BENIHYA A., AINES L., RICHAUD E., THEBAULT J., FISICARO P., DOMENEK S. and SEA PLASTICS, “Methodological development for the characterization of small microplastics and nanoplastics in seawater”, SETAC Europe 32nd Annual Meeting, Copenhagen, Denmark, 15–19 May 2022.

BESCOND A., GAIE-LEVREL F., SUTOUR C. and MACÉ T., “Development of an experimental platform for metrological performance evaluation of sensor system”, Gas Analysis 2022, Paris, France, 17-20 May 2022.

LALÈRE B. and MARLIERE F., “Monitoring pesticides in ambient air: Method harmonization based on an analytical inter laboratory comparison (ILC)”, Gas Analysis 2022, Paris, France, 17-20 May 2022.

MACÉ T., SUTOUR C., ITURRATE M., PASCALE C., BELLIRIDE S. and MONNET M., “Development of a reference standard to ensure the quality of the ammonia measurements performed in France”, Gas Analysis 2022, Paris, France, 17-20 May 2022.

MACÉ T. and WETERINGS W., “Training course accreditation”, Gas Analysis 2022, Paris, France, 17-20 May 2022.

GUIGUES N., « Évaluation des performances de capteurs dans le domaine de l’eau, Hydréos », Club eau des industriels, 24 May 2022.

GUIGUES N., « Évaluation des performances métrologiques de capteurs et analyseurs en ligne : exemple pour la mesuredu chlore libre en eau potable, Analyse & Environnement : air, eau, sols, co-produits », Journées Recherche et Industrie, Villeurbanne, France, 30-31 May 2022.

ALASONATI E., “Size distribution of extracellular vesicles in biological test samples by multidetector asymmetric flow field flow fractionation (MD-AF4)”, Journée scientifique Nantes Université « Le fractionnement flux-force en santé », Nantes, France, 5 June 2022.

DERBEZ-MORIN M., DELATOUR V., FENAILLE F., LALERE B., VASLIN-REIMANN S., HUGGETT J., BOEUF A. and BECHER F., “Multiplexed LC-HRMS/MS quantification of nine sepsis protein biomarkers in human serum”, International Mass Spectrometry Conference (IMSC) 2022, Maastricht, Netherlands, 27 August - 2 September 2022.

KOVAČIČ A., PIECHOTTA C., STEINHÄUSER L., LARDY-FONTAN S. and HEATH E., “A method for monitoring estrogens in whole surface waters by GC-MS/MS”, International Mass Spectrometry Conference (IMSC) 2022, Maastricht, Netherlands, 27 August - 2 September 2022.

LARDY-FONTAN S., PIECHOTTA C., LALERE B., LE DIOURON V., STEINHÄUSER L., HEATH E., KOVAČIČ A., POTALIVO M., DE ZORZI P., CENTIOLI D., NÄYKKI T., VIIDANOJA J., GÖKCEN T., BUDZINSKI H., LE MENACH K. and SIMON SELIH V., “A project to deliver reliable measurements of estrogens for better monitoring surveys and risk assessments”, EuChemS Chemistry Congress, Lisbonne, Portugal, 28 August – 1 September 2022.

PIECHOTTA C., LARDY-FONTAN S., LALERE B., LE DIOURON V., STEINHÄUSER L., HEATH E., KOVAČIČ A., POTALIVO M., DE ZORZI P., CENTIOLI D., NÄYKKI T., VIIDANOJA J., GÖKCEN T., BUDZINSKI H., LE MENACH K. and SIMON SELIH V., “A project to deliver reliable measurements of estrogens for better monitoring surveys and risk assessments”, EuChemS Chemistry Congress, Lisbonne, Portugal, 28 August – 1 September 2022.

ASBACH C., NOTHHELFER M., SUTTER B., WITSCHGER O., BESCOND A., GAIE-LEVREL F., RUITER S., KUIJPERS E., FRANSMAN W. and TODEA A. M., “Applicability of low cost sensors for monitoring NOAA concentrations in workplaces”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

BESCOND A., KOUST HANSEN S., THU NGUYEN Q., EGHOLM BØGH PEDERSEN P., DEBERT C., MAHNAOUI A., STABILE L., GOMEZ-MORENO F.J., ERIKSSON A., RISSLER J., ELEFTHERIADIS K., VRATOLIS S. and GAIE-LEVREL F., “Performance evaluation of black carbon portable instrument with a laboratory experimental set-up under a controlled environment”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

EGHOLM BØGH PEDERSEN P., THU NGUYEN Q., ERIKSSON A.C., RISSLER J., BESCOND A., GAIE-LEVREL F., STABILE L., GÓMEZ-MORENO F.J., VRATOLIS S., ELEFTHRIADIS K., WINKLER U., WIEDENSOHLER A., BUCAR K., MALET J. and KOUST HANSEN S., “Qualification of portable instruments for ambient aerosol measurements”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

ERIKSSON A.C., PETERSSON SJÖGREN M., SPANNE M., KRISTENSSON A., AHLBERG E., NILSSON L., EGHOLM BØGH PEDERSEN P., THU NGUYEN Q., KOUST HANSEN S., BESCOND A., GAIE-LEVREL F., DEBERT C., MAHNAOUI A., STABILE L., GÓMEZ-MORENO F.J., VRATOLIS S., TUCH T., WINKLER U., WIEDENSOHLER A. and RISSLER J., “Field intercomparison of particle counters under winter conditions”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

SPANNE M., ERIKSSON A.C., PETERSSON SJÖGREN M., ISAXON C., KRISTENSSON A., AHLBERG E., NILSSON L., SWIETLICKI E., EGHOLM BØGH PEDERSEN P., THU NGUYEN Q., KOUST HANSEN S., BESCOND A., GAIE-LEVREL F., DEBERT C., MAHNAOUI A., STABILE L., GÓMEZ-MORENO F.J., VRATOLIS S., TUCH T., WINKLER U., WIEDENSOHLER A. and RISSLER J., “Field intercomparison of stationary and portable soot instruments in a rural background environment under winter conditions”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

SUTTER B., BOIVIN A., BAU S., SIMON X., WITSCHGER O., ASBACH C., TODEA A.M., BESCOND A., GAIE-LEVREL F., RUITER S., KUIJPERS E. and FRANSMAN W., “Laboratory study of the performance of low-cost aerosol sensors with regards to reference instruments when exposed to aerosolized NOAA powders”, International Aerosol Conference (IAC 2022), Athens, Greece, 4-9 September 2022.

CAPITAINE G., STOICA D., FISICARO P. and WAGENER T., “The role of quality control into the value chain of ocean carbon observations”, ICOS Science Conference, Utrecht, The Netherlands, 15 September 2022.

EVARISTE L., GAULTIER E., CARTIER C., NOIREAUX J. , CHASSAING B., HOUDEAU E. and LAMAS B., « Mice exposed to food-grade titanium dioxide from in utero life to adulthood show sex-specific gut microbiota and metabolic disorders which are aggravated under Western-diet”, XVI th International Congress of Toxicology (ICT 2022), Maastrich, Netherlands, 18-21 September 2022.

DELATOUR V., « Importance de la métrologie et de la commutabilité pour l’Evaluation Externe de la Qualité », Club utilisateurs VITROS, Online Conference, 4 October 2022.

LARDY-FONTAN S., PIECHOTTA C., HEATH E., KOVAČIČ A., PERKOLA N., NAYKKI T., LE DIOURON V., DE ZORZI P., POTALIVO M., LE MENACH K., PARDON P., BUDZINSKI H., GOKCEN T. and LALÈRE B., “EDC-WFD: A project to deliver reliable measurements of estrogens for better monitoring survey and risks assessments of aquatic systems”, Joint IMEKO TC11 & TC24 hybrid conference 2022, Dubrovnik, Croatia, 16–19 October 2022.

VASLIN-REIMANN S., LARDY-FONTAN S., PETROV P., NAYKKI T., KOVAR P., MEERMANN B., JOTANOVIC A., KELLET M., EBERT V., ARNOLD D., PENNETTA H., CAN S., SCHNEIDER R., ŠOLC J., GUETTLER B., NOWAK A., MACÉ T., MAILLARD R. and FISICARO P., “How will the European Metrology Network POLMO support and improve pollution monitoring?”, Joint IMEKO TC11 & TC24 hybrid conference 2022, Dubrovnik, Croatia, 16–19 October 2022.

ALASONATI E., FISICARO P., LORIOT C., CHIVAS-JOLY C., ROPERS M.-H., COULIBALY C. A., BREYSSE C., BENIHYA A., DOMENEK S. and SEA PLASTICS, « Les activités LNE sur la caractérisation de nano et micro plastiques au niveau français et européen : Projets Microplast, Moustic et PlasticTrace », Comité Scientifique et Technique « ITAI ACTIA», Paris, France, 25 Novembre 2022.

GUIGUES N. and VILLEMAGNE E., « Valider les outils innovants de mesure dans le domaine de l’eau : pourquoi et comment Dream », GT métrologie, 29 November 2022.

DELATOUR V., “How to improve the contribution of metrology institutes to medical laboratory standardization ?”, Congrès CIRME “Implementation of metrological traceability in laboratory medicine: where we are and what is missing?”, Milan, Italy, 30 November 2022.

ALASONATI E., CAEBERGS T., PÉTRY J., SEBAÏHI N., FISICARO P. and FELTIN N., “Size measurement of silica nanoparticles by Asymmetric Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering: a comparison exercise between two metrological institutes”, Journal of Chromatography A, 2021, 1638 (Special issue), 461859, DOI: 10.1016/j.chroma.2020.461859.

BASTKOWSKI F., SANDER B., LOZANO H., PUELLES M., SNEDDEN A., DELEEBEECK L., ASAKAI T., HWANG E., JO K., ORTIZ-APARICIO J. L., MONTERO-RUIZ J., ROZIKOVÁ M., KOZLOWSKI W., QUEZADA H. T., MORALES L. V., AHUMADA D. A., BORGES P. P., NEVES R. S., SOBRAL S. P., UYSAL E., LIV L., PROKUNIN S., DOBROVOLSKYI V., STOICA D., WU B., MA L., MÁRIÁSSY M., HANKOVÁ Z., SOBINA A., and SHIMOLIN A., “Key comparison CCQMK73.2018 Amount content of H+ in Hydrochloric acid (0.1 mol.kg-1)”, Metrologia, 2021, 58, Technical supplement, 1A, 08002, DOI: 10.1088/0026-1394/58/1A/08002.

BESCOND A., OSTER C., FISCARO P., GODDARD S., QUINCEY P., TSAKANIKA L.-A., LYMPEROPOULOU T. and OCHSENKUHN-PETROPOULOU M., “Method for preparation of a candidate reference material of PM10 and PM2.5 airborne particulate filters loaded with incineration ash-Inter comparison results for metal concentration”, Atmosphere, 2021, 12, 67, DOI: 10.3390/atmos12010067.

BETTENCOURT DA SILVA R.J.N., SAAME J., ANES B., HEERING A., LEITO I., NAYKKI T., STOICA D., DELEEBEECK L., BASTKOWSKI F., SNEDDEN A. and CAMOES M.F., “Evaluation and validation of detailed and simplified models of the uncertainty of unified pH abs H₂O measurements in aqueous solutions”, Analytica Chimica Acta, 2021, 1182,1182 338923, DOI: 10.1016/j.aca.2021.338923.

BOURROUS S., BARRAULT M., MOCHO V., POIRIER S.,OUF F.-X., BARDIN-MONNIER N., CHARVET A., THOMAS D., BESCOND A., FOUQUEAU A., MACÉ T. and GAIE-LEVREL F., “A performance evaluation and inter-laboratory comparison of community face coverings in the context of COVID-19 pandemic”, Aerosol and Air Quality Research, 2021, 21, 6, DOI: 10.4209/aaqr.200615.

BUCHTA C., CAMP J.V., JOVANOVIC J., CHIBA P., PUCHHAMMER-STÖCKL E., MAYERHOFER M., PLICKA H., LERCHER A., POPA A.M., ENDLER L., BERGTHALER A., HUF W., BENKA B., DELATOUR V., MÜLLER M.M., GRIESMACHER A., ABERLE S.W. and GÖRZER I., “The versatility of external quality assessment for the surveillance of laboratory and in vitro diagnostic performance: SARS-CoV-2 viral genome detection in Austria”. Clin Chem Lab Med., 2021, 59, 10, 1735-1744, DOI: 10.1515/cclm-2021-0604.

COBBAERT C.M., ALTHAUS H., BEGCEVIC BRKOVIC I., CEGLAREK U., COASSIN S., DELATOUR V., DEPREZ L., DIKAIOS I., DITTRICH J., HOOFNAGLE A.N., KOSTNER G.M., KRONENBERG F., KUKLENYIK Z., PRINZING U., VESPER H.W., ZEGERS I. and RUHAAK L.R.,”Towards an SI-Traceable Reference Measurement System for Seven Serum Apolipoproteins Using Bottom-Up Quantitative Proteomics: Conceptual Approach Enabled by Cross-Disciplinary/Cross-Sector Collaboration”, Clin Chem., 2021, 67, 3, 478-489, DOI: 10.1093/clinchem/hvaa239.

DELEEBEECK L., SNEDDEN A., NAGY D., NAGYNÉ SZILÁGYI Z., ROZIKOVÁ M., VIČAROVÁ M., HEERING A., BASTKOWSKI F., LEITO I., QUENDERA R., CABRAL V. and STOICA D., “Unified pH measurements of ethanol, methanol, and acetonitrile, and their mixtures with water”, Sensors, 2021, 21, 3935, DOI: 10.3390/s21113935.

FIGTREE G.A., VERNON S.T., HADZIOSMANOVIC N., SUNDSTRÖM J., ALFREDSSON J., ARNOTT C., DELATOUR V., LEÓSDÓTTIR M and HAGSTRÖM E. “Mortality in STEMI patients without standard modifiable risk factors: a sex-disaggregated analysis of SWEDEHEART registry data”, Lancet, 2021, 397, 10279, 1085-1094, DOI: 10.1016/S0140-6736(21)00272-5.

HAMDAOUI Q., BENCSIK A., FLAMANT F., DELCOUR S., MACÉ T., VASLIN-REIMANN S. and GAIE-LEVREL F., “Metrological characterization of an aerosol exposure chamber to explore the inhalation effects of the combination of paraquat and TiO2 nano-objects”, Aerosol and Air Quality Research, 2021, 21, 8, DOI: 10.4209/aaqr.200626.

HAMDAOUI Q., ZEKRI Y., RICHARD S., AUBERT D., GUYOT R., MARKOSSIAN S., GAUTHIER K., GAIE-LEVREL F., BENCSIK A. and FLAMANT F., “Prenatal exposure to paraquat and nanoscaled TiO₂ aerosols alters the gene expression of the developing brain”, Chemosphere, 2022, online: 16 Sept. 2021, 287, DOI: 10.1016/j.chemosphere.2021.132253.

HOOFNAGLE A.N., COBBAERT C.M., DELATOUR V., KELLEHER N.L., LOWENTHAL M.S. and SHUFORD C.M., “Should LC-MS/MS Be the Reference Measurement Procedure to Determine Protein Concentrations in Human Samples?”, Clin Chem., 2021, 67, 3, 466-471, DOI: 10.1093/clinchem/hvaa256.

HORENDER S., AUDERSET K., QUINCEY P., SEEGER S., NIELSEN SKOV S., DIRSCHERL K., SMITH T.O.M., WILLIAMS K., AEGERTER C.C., KALBERMATTER D.M., GAIE-LEVREL F. and VASILATOU K., “Facility for production of ambient-like model aerosols (PALMA) in the laboratory: application in the intercomparison of automated PM monitors with the reference gravimetric method”, Atmos. Meas. Tech., 2021, 14, 1225-1238, DOI: 10.5194/amt-14-1225-2021.

HUYNH H.H., BOEUF A., DERBEZ-MORIN M., DUPUY A.M., LALERE B., DELATOUR V. and VINH J., “Development of an antibody-free ID-LC MS method for the quantification of procalcitonin in human serum at sub-microgram per liter level using a peptide-based calibration”, Anal Bioanal Chem., 2021, 413, 19, 4707-4725, DOI: 10.1007/s00216-021-03361-0.

HUYNH H.H., BOEUF A., DERBEZ-MORIN M., DUPUY A.M., LALERE B., VINH J. and DELATOUR V., “Response to Letter to the Editor regarding, “Development of an antibody-free ID-LC MS method for the quantification of procalcitonin in human serum at sub-microgram per liter level using a peptide-based calibration”, Anal Bioanal Chem., 2021, 413, 19, 4921-4922, DOI: 10.1007/s00216-021-03459-5.

HUYNH H.H., BOEUF A., PFANNKUCHE J., SCHUETZ P., THELEN M., NORDIN G., VAN DER HAGEN E., KAISER P., KESSELER D., BADRICK T., POGGI B., TIIKKAINEN U., DAVIES G.J., KESSLER A., PLEBANI M., VINH J. and DELATOUR V., “Harmonization status of procalcitonin measurements: what do comparison studies and EQA schemes tell us?”, Clin Chem Lab Med., 2021, 59, 10, 1610-1622, DOI: 10.1515/cclm-2021-0566.

HUYNH H.H., BOEUF A., VINH J. and DELATOUR V., “Evaluation of the necessity and the feasibility of the standardization of procalcitonin measurements: Activities of IFCC WG-PCT with involvement of all stakeholders”, Clin Chim Acta, 2021, 515, 111-121, DOI: 10.1016/j.cca.2021.01.004.

KOLEVA B., DIMITROVA L., STOICA D. and FISICARO P., “Application of secondary pH measurement method for homogeneity and stability assessment of reference materials”, Accreditation and Quality Assurance, 2021, 26, 113–120, DOI: 10.1007/s00769-021-01464-w.

JEONG J.S., YIM Y.H., LIU Q., YONG S., LIU H., BELTRÃO B., SCAPIN S., SADE Y., GARRIDO B., BOEUF A., DELATOUR V., KILPATRICK E., LOWENTHAL M., MI W., WU L., WANG J., KINUMI T., HENRION A., OHLENDORF R., OZTUG M. and AKGOZ M., “Protein Quantification Purity-Assessed Recombinant Protein Contents in Buffer Solution using Insulin analogue - CCQMK151”, Metrologia, 2021, 58, Tech. Suppl., 08007, DOI: 10.1088/0026-1394/58/1A/08007.

LAINELA S., CAPITAINE G., HEERING A., LEITO I., AGNES B., CAMOES F. and STOICA D., “Toward Unified pH of Saline Solutions”, Water, 2021, 13, 2522, DOI: 10.3390/w13182522.

MATHON B., DABRIN A., ALLAN I., LARDY-FONTAN S., TOGOLA A., GHESTEM J-P., TIXIER C., GONZALEZ JL., ALASONATI E., FERREOL M., DHERRET L., YARI A., RICHARD L., MOREIRA A., EON M., DELEST B., POLLONO C., MUNSCHY C., NOEL-CHERY E., EL MOSSAOUI M., STAUB P-F., MAZZELLA N. and MIEGE C., « Les échantillonneurs intégratifs passifs, des outils pertinents pour améliorer la surveillance règlementaire de la qualité chimique des milieux aquatiques ? », TSM, 2021, 6, 57-71, DOI: 10.36904/tsm/202106057.

MI W., JOSEPHS R.D., MELANSON J.E., DAI X., WANG Y., ZHAI R., CHU Z., FANG X., THIBEAULT M-P., STOCKS B.B., MEIJA J., BEDU M., MARTOS G., WESTWOOD S., WIELGOSZ R.I., LIU Q., TEO T.L., LIU H., J TAN Y., ÖZTUĞ M., SABAN E., KINUMI T., SAIKUSA K., SCHNEIDER R.J., WELLER M.G., KONTHUR Z., JAEGER C., QUAGLIA M., MUSSELL C., DRINKWATER G., GIANGRANDE C., VANEECKHOUTTE H., BOEUF A., DELATOUR V., LEE J.E., O'CONNOR G., OHLENDORF R., HENRION A., BELTRÃO P.J., NARESSI SCAPIN S.M. and SADE Y.B., “PAWG Pilot Study on Quantification of SARS-CoV-2 Monoclonal Antibody - Part 1”, Metrologia, 2022, published: 13 Dec. 2021, 59,1A, DOI: 10.1088/0026-1394/59/1A/08001.

MILLER G. W., GREENBERG N., BUDD J. and DELATOUR V., “The evolving role of commutability in metrological traceability”, Clin Chim Acta., 2021, 514, 84-89, DOI: 10.1016/j.cca.2020.12.021.

MIRMONT E., BOEUF A., CHARMEL M., VASLIN-REIMANN S., LALERE B., LAPRÉVOTE O. and LARDY-FONTAN S., “Development and implementation of an analytical procedure for the quantification of natural and synthetic steroid hormones in whole surface waters”, Journal of Chromatography B, 2021, 15, 1175, DOI: 10.1016/j.jchromb.2021.122732.

NOIREAUX J., LOPEZ-SANZ S., VIDMAR J., CORREIA M., DEVOILLE L., FISICARO P. and LOESCHNERK., “Titanium dioxide nanoparticles in food: comparison of detection by triple-quadrupole and high resolution ICP-MS in single particle mode”, Journal of nanoparticle research, 2021, 23, 102, DOI: 10.1007/s11051-021-05198-1.

PANTEGHINI M., BRAGA F., CAMARA J.E., DELATOUR V., VAN UYTFANGHE K., VESPER H.W., ZHANG T. and JCTLM Task Force on Reference measurement system implementation, “Optimizing available tools for achieving Result standardization: Value added by Joint Committee on Traceability in Laboratory Medicine (JCTLM)”, Clin Chem., 2021, 67, 12, DOI: 10.1093/clinchem/hvab178.

RADTKE V., STOICA D., LEITO I., CAMÕES F., KROSSING I., ANES B., ROZIKOVÁ M., DELEEBEECK L., VELTZ´E S., NÄYKKI T., BASTKOWSKI F., HEERING A., DÁNIEL N., QUENDERA R., LIV L., UYSAL E. and LAWRENCE N., “A unified pH scale for all solvents Part I - Intention and reasoning (IUPAC Technical Report)”, Pure and Applied Chemistry, 2021, 93, 9, 1049-1060, DOI: 10.1515/pac-2019-0504.

REGNIER R., AVRIN G., BARBOSA V., BOFFETY D., KALOUGUINE A. and LARDY-FONTAN S., « Validation de méthodologies d’évaluation de solutions de désherbage autonomes, dans le cadre des projets Challenge ROSE et METRICS », Revue Ouverte d'Intelligence Artificielle, 2021, 2, 1, 11-32, DOI: 10.5802/roia.8.

SEEGER S., OSAN J., OTTÓ CZÖMPÖLY O., GROSS A., STOSNACH H., STABILE L., OCHSENKUEHN-PETROPOULOU M., LAMPRINI-ARETI T., LYMPEROPOULOU T., GODDARD S., FIEBIG M., GAIE-LEVREL F., KAYSER Y. and BECKHOFF B., “Quantification of element mass concentrations in ambient aerosols by combination of cascade impactor sampling and mobile total reflection X-ray fluorescence spectroscopy”, Atmosphere, 2021, 12, 309, DOI: 10.3390/atmos12030309.

SEITZ S., ROZIKOVA M., VICAROVA M., THIRSTRUP C., WANG H. and STOICA D., “EURAMET Comparison 1462 Supplementary CCQM comparison Euramet.QM-S12 Electrolytic conductivity at pure water level - Final Report”, Metrologia, 2021, 58, Techn. suppl. 1A, 08001, DOI: 10.1088/0026-1394/58/1A/08001.

STOICA D., ANES B., FISICARO P. and FILOMENA CAMÕES M., “Feasibility of multifunction calibration of H+ responsive glass electrodes in seawater”, Pure and Applied Chemistry, 2021, 93, 12, 1487-1497, DOI: 10.1515/pac-2020-0202.

BESCOND A., GAIE-LEVREL F., SUTOUR C. and MACE T., « Protocole expérimental pour caractériser les performances des capteurs à bas coût pour la surveillance de la qualité de l'air dans un environnement contrôlé », 34e Congrès Français sur les Aérosols, Paris, France, 26-28 janvier 2021.

BOURROUS S., THOMAS D., CHARVET A., MONNIER N., FOUQUEAU A., BESCOND A., GAIE-LEVREL F. and OUF F-X., « Caracterisation des performances des masques barrière à usage non sanitaire : intercomparaison des protocoles et premiers enseignements », 34e Congrès Français sur les Aérosols, DOI: 10.25576/ASFERA-CFA2021-24841, Paris, France, 26-28 janvier 2021.

SUTTER B., BAU S., SIMON X., PAYET R., BOIVIN A., WITSCHGER O., BESCOND A. and GAIE-LEVREL F., « NOAA LCS : Un projet de recherche prénormative sur l'application des microcapteurs d'aérosols pour la mesure des NOAA sur les lieux de travail », 34e Congrès Français sur les Aérosols, Paris, France, 26-28 janvier 2021.

MATHON B., DABRIN A., ALLAN I., LARDY-FONTAN S., TOGOLA A., GHESTEM J-P., TIXIER C., GONZALEZ JL., FERREOL M., DHERRET L., YARI A., RICHARD L., MOREIRA A., EON M., DELEST B., NOEL-CHERY E., EL MOSSAOUI M., ALASONATI E., STAUB P-F., MAZZELLA N. and MIEGE C., « Présentation résultats DGT, POCIS, SR et incertitudes », Colloque restitution finale RSP, Lyon-Villeurbanne, France, mars 2021.

FOUQUEAU A., GAIE-LEVREL F., BESCOND A., VASLIN-REIMANN S. and MACE T., « Masques de protection respiratoire : du contexte normatif aux travaux de comparaison inter laboratoire pour les mesures d’efficacité de filtration », Webinaire de la Société Française des Séparations Fluides Particules, Visioconférence, 1er avril 2021.

FISICARO P., “Metrology for Ocean sciences: The European Metrology Network for Climate and Ocean Observation”, Eurogoos conference 2021, Online conference, 3-5 mai 2021.

BENCSIK A., HAMDAOUI Q., NOIREAUX J., FISICARO P., GADOT N., FLAMANT F. and GAIE-LEVREL F., “An inhalation study combining nano-TiO₂ and paraquat exposure in a transgenic mouse model of parkinson's disease: a novel approach to study nanopesticides impact on brain function”, NeuroFrance 2021, Colloque virtuel international, 19-21 mai 2021.

COBBAERT C., BEGCEVIC-BRKOVIC I., DITTRICH J., KUKLENYIK Z., CEGLAREK U., ALTHAUS H., ANGLES-CANO E., COASSIN S., DELATOUR V., DEPREZ L., DIKAIOS I., HOOFNAGLE A., KOSTNER G., KRONENBERG F., PRINZING U., VESPER H. and RUHAAK R., “In transition to the next generation reference materials and reference measurement procedures for apolipoprotein standardization”, 89^th European Atherosclerosis Society (EAS) Congress, Helsinki, Finlande, Proceedings: Atherosclerosis, 2021, 331, 201-e202, 10.1016/j.atherosclerosis.2021.06.618, Online Congress, 30 May – 2 June 2021.

LAINELA S., STOICA D., LEITO I., HEERING A., ANES B. and FILOMENA CAMÕES M., “How reliable is your pH data?”, ASLO, Online meeting, 22-27 juin 2021.

DELATOUR V., “Potential contribution of the EMN TraceLabMed to the HALMA project harmonization of measurands in laboratory medicine through data aggregation of EQA Data”, Online conference, 23 juin 2021.

FISICARO P., “Brief presentation of EURAMET and the research programmes in metrology”, Workshop on Standardization in extracellular vesicles metrology, Paris, France, 25 juin 2021.

STEINHÄUSER L., LARDY-FONTAN S., PIECHOTTA C., HEATH E., PERKOLA N., BALZAMO S., COTMAN M., GÖKCEN T., GARDIA-PAREGE C., BUDZINSKI H., LALERE B., “Metrology for monitoring of endocrine disrupting chemicals under the EU water framework directive”, Goldschmidt2021, Online conference, 4-9 juillet 2021.

DELATOUR V., “Reference methods and commutable reference materials for standardization of immunoassays”, IFCC-Snibe International Symposium on Immunoassay-Based Diagnostics, Online conference, 7-8 Juillet 2021.

QUAGLIA M., CANO S., FILLMER A., FLOEL A. and GIANGRANDE C., “The NeuroMET project: Metrology and innovation for early diagnosis and accurate stratification of patients with neurodegenerative diseases”, 2021 Alzheimer's Association International Conference (AAIC 2021), Proceedings: Alzheimer's & dementia, 2021, 17, S5, e053655, DOI: 10.1002/alz.053655, Denver, Colorado, USA, 26-30 July 2021.

BESCOND A., GAIE-LEVREL F. and MACÉ T., “Experimental set-up for low cost sensor system performance assessment in a controlled environment”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021

BOEUF A., MIRMONT E., CHARMEL M., LAPREVOTE O., VASLIN-REIMANN S., LALERE B. and LARDY-FONTAN S., “Towards a reliable quantification of natural steroids and analogous pharmaceuticals at ultratrace level in water and human matrix”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

DELATOUR V., « La R&D en métrologie pour assurer la fiabilité des examens de biologie médicale », 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

DERBEZ-MORIN M., DELATOUR V., FENAILLE F., LALERE B., HUGGET J., BOEUF A. and BECHER F., “Development of a LC-HRMS method for the multiplexed quantification of nine sepsis protein biomarkers in human serum”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

FOUQUEAU A., GAIE-LEVREL F., MACE T., VASLIN-REIMANN S.and BESCOND A., « Face mask filtration efficiency: a characterisation of measurement protocol and data analysis », 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

GIANGRANDE C., BOEUF A., VANEECKOUTTE H., HUYNH H.H., LALÈRE B., VASLIN-REIMANN S., VINH J., HIRTZ C., SWART C., HUGGETT J., QUAGLIA M. and DELATOUR V., “Purity analysis of primary calibrators for absolute quantitation of clinically relevant proteins: state-of-the-art and perspectives”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

LALERE B., GANTOIS F., PHILIPP R. and VASLIN-REIMANN S., “Alcoref Project: certified reference materials for breath alcohol control - An improvement in European metrological infrastructure for road safety”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

LARDY-FONTAN S., “ONE HEALTH from the initial concept to the future challenges: Metrology as a cornerstone?”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

LARDY-FONTAN S., PIECHOTTA C., HEATH E., PERKOLA N., BALZAMO S., CENTIOLI D., GARDIA-PARÈGE C., BUDZINSKI H., COTMAN M., GOKCEN T. and LALÈRE B., “EDC-WFD: a project to deliver reliable measurements of estrogens for better monitoring survey and risks assessments of aquatic systems”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

MACÉ T. and SUTOUR C., “Air pollution monitoring: Development of ammonia (NH3) reference standards at nmol/mol levels”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

MAILLARD R., “Creation of a new European Metrology Network on Pollution Monitoring (POLMO) inside EURAMET”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

MARCHAND C., SPINELLE L. and MACÉ T., “A first certification protocol for the evaluation of sensor systems dedicated to the ambient air quality monitoring”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

STOICA D., “Realization of a universal pH scale: a step forward for measuring acidity and achieve pH comparability in any media”, 20^e Congrès international de métrologie (CIM 2021), Lyon, France, 7-9 sept. 2021.

DELATOUR V., “Activities of IFCC Working group on standardization of procalcitonin assays with involvement of all stakeholders”, SeptiMet Stakeholders meeting, Online conference, 14 Sept. 2021.

STOICA D., « Rôle des laboratoires nationaux de métrologie dans l’évaluation et la quantification de l’acidification des eaux marines », Atelier SOMLIT, Brest, France, 15 septembre 2021.

SEEGER S., OSAN J., CZÖMPÖLY O., GROSS A., STOSNACH H., STABILE L., OCHSENKUEHN-PETROPOULOU M., TSAKANIKA L. A., LYMPEROPOULOU T., GODDARD S., FIEBIG M., GAIE-LEVREL F., KAYSER Y. and BECKHOFF B., “Element mass concentrations in ambient aerosols, a comparison of results from filter sampling / ICP-MS and cascade impactor sampling / Mobile total reflection X-ray fluorescence spectroscopy”, 12^th International Conference on “Instrumental Methods of Analysis, Online conference, 20-23 Sept. 2021.

MATHON B., DABRIN A., ALLAN I., LARDY-FONTAN S., TOGOLA A., GHESTEM J-P., TIXIER C., GONZALEZ JL., FERREOL M., DHERRET L., YARI A., RICHARD L., MOREIRA A., EON M., DELEST B., NOEL-CHERY E., EL MOSSAOUI M., ALASONATI E., STAUB P-F., MAZZELLA N. and MIEGE C., « Evaluation de la pertinence des EIP pour la surveillance réglementaire des milieux aquatiques », ASTEE, Paris, France, 28-30 septembre 2021.

FISICARO P., “Metrology for Ocean Sciences: The European Metrology Network for Climate and Ocean Observation”, MetroSea, Online conference, 4-6 octobre 2021.

STOICA D., “Implementation of documentary standards in support of international and European climate strategies”, MetroSea, Online conference, 4-6 octobre 2021.

STOICA D., FISICARO P., BASTKOWSKI F., SEITZ S. and SALVETAT F., “What place for the National Metrology Institutes in the Ocean observing system? An example for assessing and quantifying ocean acidification”, MetroSea, Online conference, 4-6 octobre 2021.

DERBEZ-MORIN M., BOEUF A., FENAILLE F., LALERE B., HUGGETT J., DELATOUR V. and BECHER F., « Développement d’une méthode LC-HRMS/MS pour la quantification multiplexée de huit biomarqueurs protéiques du sepsis dans le sérum humain », SEP 2021, Paris, France, 5-7 octobre 2021.

ALASONATI E., CAEBERGS T., PÉTRY J., SEBAÏHI N., FISICARO P. and FELTIN N., “Comparison exercise between two metrological institutes for size measurement of silica nanoparticles by Asymmetric Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detector”, Virtual Symposium on Field- and Flow-based Separations 2021, Online conference, 11-13 octobre 2021.

ALASONATI E., « Evaluation de méthodes d’échantillonnage et de caractérisation de particules polymères disséminées dans le milieu marin », Forum Emballages LNE, Online conference, 12 octobre 2021.

FISICARO P., “Primary methods for chemical analysis and their applications at LNE”, Séminaire 60 ans CETAMA, Nîmes, France, 19-21 octobre 2021.

GUIGUES N., « Guide Eurachem - L’échantillonnage dans le processus de mesure- Apport d’exercices d’intercomparaison », Séminaire 60 ans CETAMA, Nîmes, France, 19-21 octobre 2021.

MATHON B., DABRIN A., TOGOLA A., GHESTEM J-P., ALLAN I., LARDY-FONTAN S., YARI A., FERREOL M., DHERRET L., EL MOSSAOUI M., TIXIER C., NOEL-CHERY E., MUNSCHY C., POLLONO C., ALASONATI E., GONZALEZ J-L., MAZZELLA N. and MIÈGE C., “Relevance and limitation of integrative passive samplers (IPS) in support of surface waters monitoring: results of a French national-scale study”, SETAC, Portland, USA, 14-18 novembre 2021.

DELATOUR V., “The importance of demonstrating commutability of reference materials with IQC”, EuroMedLab - Symposium Advances in IQC tools and techniques, Munich, Allemagne, 28 Nov. - 2 Dec. 2021.

FISICARO P., “Development of scientific and technical capabilities in the field of chemical analysis”, EURAMET Preparatory Workshop: 2022 Call for Research Potential Projects, Online conference, 7-9 décembre 2021.

For some thirty years, endocrine disruption has become a central concern at the political, regulatory, societal and scientific levels. This is due to the fact that, in the field of the environment, endocrine-disrupting compounds (EDCs), and among them, natural steroid hormones and related compounds (analogous pharmaceuticals), can exert harmful effects at concentrations below the ng.L^-1 and pose a serious threat to biodiversity. In the field of human health, these compounds are widely used for the diagnosis and treatment monitoring of various diseases and disorders. The aim of this thesis was notably to ensure, in both fields, the reliable quantification of endocrine disruptors and/or biomarkers to support effective decision-making at relevant concentration levels. First, 23 steroid hormones and related compounds were selected following an extensive bibliographical work that highlighted their common interest in both fields. Then, multiresidue quantification methods by LC-MS/MS were developed in whole continental waters and human serum. Limits of quantification in the ng.L^-1 and the ng.mL^-1 range were respectively obtained in the field of the environment and the field of human health. Measurement uncertainty was also assessed, enhancing the degree of confidence in measurement results. Implementing the methods allowed the characterisation of many target compounds in surface waters with some of them very few documented in France and Europe. Also, traceability of measurement results to the SI was established. Present work represents a significant step towards the development of multiresidue reference methods and future certification of reference materials, which are actually limited or missing.

Health, Environment, Steroid hormone, Whole water, Human serum, Ultra-trace level, Multiresidue method, Liquid Chromatography coupled to Mass Spectrometry, Quality of data, Metrology

Procalcitonin (PCT) measurement is a usefully tool to aid the early diagnosis and antibiotic stewardship for patients with sepsis. As sepsis diagnosis and antibiotic stewardship rely on fixed clinical cutoffs (threshold concentration values), ensuring accuracy and comparability of PCT results over time and space is essential to ensure an appropriate diagnostic and therapeutic management.

This thesis consisted in studying the metrological traceability of these methods of PCT measurement. The work initially consisted in documenting the current state for results’ comparability between the different commercially available immunoassays for PCT. The evaluation of correlation studies and external quality assessment schemes results indicate that properly evaluating the current state of result’s comparability and discriminating the origin of result’s discrepancies is made difficult because studies were conducted in non-harmonized conditions and commutability of the materials was not characterized. Thus, it seems essential to conduct quality external evaluation using commutable control materials. In order to evaluate accuracy of commercially available immunoassays for PCT and to meet the regulatory requirements for the metrological traceability of results, a candidate reference method based on isotopic dilution liquid chromatography coupled to mass spectrometry was developed and validated for an absolute quantification of PCT in human serum with a limit of quantification of 0.25 ng/mL. Results traceability to the SI units could be established thanks to a thorough purity assessment of the primary standards by amino acid analysis and correction of impurities contribution.

procalcitonin, sepsis, antibiotic resistance, isotope dilution liquid chromatography coupled mass spectrometry, reference method, metrological traceability

The asymmetrical flow field flow (AF4) is a technique used to measure the size of nanoparticles. It consists in  fractionating the different populations inside the sample as a function of their hydrodynamic diameter. In the 1960s, a model (thereafter called classical model) has been developed, relying the retention time of a nanoparticle with its hydrodynamic diameter. However, the model validity is based on work hypothesizes which are not always respected depending on the experimental conditions. The work of this PhD thesis has consisted firstly in the study of the mechanisms governing the nanoparticles retention inside the AF4 channel. Notably it has been shown that interactions between the nanoparticles and the accumulation wall of the AF4 biased the results predicted by the classical model. Another model (thereafter called p-w model), which takes electrostatic and Van de Walls interactions into account, has been developed. Tests realized with particle standards for size showed that the p-w model give results with a better trueness than the classical model and that it can be applied for a larger range of experimental conditions. A validation of this model has been conducted and an uncertainty budget has been developed by following the Monte Carlo method. The metrological traceability of the measurement results has also been demonstrated.

AF4, nanoparticles, FFF theory, method validation

PhD thesis: https://pastel.archives-ouvertes.fr/tel-03320831/document